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KMID : 0350519950480041099
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Journal of Catholic Medical College
1995 Volume.48 No. 4 p.1099 ~ p.1110
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Effects of Epidermal Growth Factor on the Wound Healing after Excimer Laser Photokeratectomy in Rabbits
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Abstract
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Excimer laser has been recently used in correcting myopia, removing superficial corneal opacity and making a reproducible experimental corneal wound in ophthalmologic field. The corneal wound healing after excimer laser photokeratectomy showed a
little
difference from that after conventional surgical keratectomy. However the corneal epithelium is important in the wound healing after excimer laser photokeratectomy in preventing the postoperative corneal haze.
Epidermal growth factor (EGF) has been believed to promote the corneal epithelial wound healing by stimulating the proliferation of the epithelial cells located at the wound base and limbus.
We made transepithelial photokeratectomy (5.0 mm ablation zone, total 250 pulses) by excimer laser (Summit Technology, Inc., ExciMed UV 200 LA, USA) n 16 rabbits to compare the rate of corneal wound healing and the histological findings between
the
group received only photokeratectomy (control) and the groups received photokeratectomy and topical EGF treatement (10, 100, and 1000㎍/g, every 12 hrs, 3x, for 36 hrs). The status of wound was observed with a slitlamp biomicroscope and the rate
of
wound closure was evaluated by using morphometry. The histological findings of cell proliferation were examined by hematoxylin-eosin and immunohistochemical staining for proliferating cell nuclear antigen (PCNA).
@EN The results were as follows :
@EN 1. The corneal epithelial wounds by excimer laser photokeratectomy treated by EGF was closed significantly faster than those not treated by EGF. Thfere was no significant differene of wound healing rate among three groups treated with
different
concentrations of EGF.
2. PCNA immunoreactive cells were appeared at the basal cells were appeared at the basal cell layer of limbal and wound area in EGF-treated groups and no PCNA immunoreactivity was detected in control group at 36 hours after excimer laser
photokeratectomy.
These results suggest that epidermal grouwth factor accelerates the corneal epithelial wound healing after excimer laser photokeratectomy by stimulating the proliferation of the basal cells of limbal and wound area and results in a faster wound
closure-rate.
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